1
00:00:04,460 --> 00:00:04,960
OK.

2
00:00:04,960 --> 00:00:08,070
We're going to get a little
bit more detail about how

3
00:00:08,070 --> 00:00:09,500
to sharpen images.

4
00:00:09,500 --> 00:00:13,010
Here's microfluidic
devices that we saw before

5
00:00:13,010 --> 00:00:15,230
in the flatbed scanner section.

6
00:00:15,230 --> 00:00:19,380
I cropped a larger
picture, and as we zoom in,

7
00:00:19,380 --> 00:00:22,390
we're actually seeing
some Newton rings, which

8
00:00:22,390 --> 00:00:25,480
we're not loving, but let's
pretend that they're not there.

9
00:00:25,480 --> 00:00:29,550
You know that we're going to get
Newton rings when you put glass

10
00:00:29,550 --> 00:00:33,370
on top of glass,
or-- this is PDMS,

11
00:00:33,370 --> 00:00:36,090
which is also going to
give us some Newton rings.

12
00:00:36,090 --> 00:00:42,420
So let's just take an area,
maybe around here, just

13
00:00:42,420 --> 00:00:44,330
to pay attention.

14
00:00:44,330 --> 00:00:45,950
I'm going to zoom
in a little more.

15
00:00:49,130 --> 00:00:54,990
Your eye should focus around
here and around the numbers,

16
00:00:54,990 --> 00:00:56,920
and maybe over here.

17
00:00:56,920 --> 00:01:00,520
To see what happens when we
sharpen, we go to the Filter,

18
00:01:00,520 --> 00:01:04,310
we go down to Sharpen,
and use Unsharp Mask.

19
00:01:04,310 --> 00:01:06,920
There are all kinds of
things, but for our purposes,

20
00:01:06,920 --> 00:01:09,160
Unsharp Mask is the way to go.

21
00:01:09,160 --> 00:01:12,710
And it was already set
at 128 once before.

22
00:01:12,710 --> 00:01:16,440
Let's go down to almost
very little sharpening,

23
00:01:16,440 --> 00:01:19,150
almost kind of the
original image.

24
00:01:19,150 --> 00:01:23,630
And watch as I increase
the amount of sharpening.

25
00:01:23,630 --> 00:01:28,520
You're seeing more and
more getting sharp.

26
00:01:28,520 --> 00:01:33,280
But, unfortunately, what also
happens is you get a lot of,

27
00:01:33,280 --> 00:01:35,930
the dirt is sharpening as well.

28
00:01:35,930 --> 00:01:40,560
And we'll discuss that in
a moment, a little bit.

29
00:01:40,560 --> 00:01:45,050
But we're going to go at
length about cleaning images.

30
00:01:45,050 --> 00:01:49,430
For example, if I accepted this
with 228, which is considerably

31
00:01:49,430 --> 00:01:56,630
sharper than before-- let
me undo it with Command-Z.

32
00:01:56,630 --> 00:01:59,160
That was without sharpening.

33
00:01:59,160 --> 00:02:01,290
That's with sharpening.

34
00:02:01,290 --> 00:02:02,560
I hope you're seeing.

35
00:02:02,560 --> 00:02:04,560
Look over here now.

36
00:02:04,560 --> 00:02:06,780
Without sharpening.

37
00:02:06,780 --> 00:02:07,930
With sharpening.

38
00:02:07,930 --> 00:02:10,669
So we're getting some
real clarity here,

39
00:02:10,669 --> 00:02:12,630
which I think is
what we want to show.

40
00:02:12,630 --> 00:02:15,710
But, as I said, we are
also getting some junk,

41
00:02:15,710 --> 00:02:19,730
which, if I decided
to get rid of,

42
00:02:19,730 --> 00:02:21,940
let's say with
the Healing Brush.

43
00:02:21,940 --> 00:02:23,390
Little bit here.

44
00:02:23,390 --> 00:02:24,730
Get rid of this.

45
00:02:24,730 --> 00:02:26,320
Get rid of this.

46
00:02:26,320 --> 00:02:28,760
This is making
the image cleaner.

47
00:02:28,760 --> 00:02:31,840
And the question is, am
I permitted to do this?

48
00:02:31,840 --> 00:02:37,510
And we're going to have a very
important deeper discussion

49
00:02:37,510 --> 00:02:42,810
about how much we can, in fact,
enhance scientific images.

50
00:02:42,810 --> 00:02:46,350
For the time being,
let's leave it at this.

51
00:02:46,350 --> 00:02:51,310
And we're going
to save the image

52
00:02:51,310 --> 00:02:56,060
and clearly indicate
this has been sharpened.

53
00:02:56,060 --> 00:02:58,380
And that's it.

54
00:02:58,380 --> 00:03:01,140
There you go.