1 00:00:04,340 --> 00:00:07,390 Creating images that are more than good enough 2 00:00:07,390 --> 00:00:10,610 is what this case study is about. 3 00:00:10,610 --> 00:00:13,310 This happened quite a while ago, about-- I can't 4 00:00:13,310 --> 00:00:15,640 believe it's 20 years ago. 5 00:00:15,640 --> 00:00:19,800 In this lab, this is the image that the researchers came up 6 00:00:19,800 --> 00:00:21,860 with on their own. 7 00:00:21,860 --> 00:00:29,180 You're seeing a surface that has etched-out hydrophobic lines. 8 00:00:29,180 --> 00:00:32,320 And so when you drop water on the surface, 9 00:00:32,320 --> 00:00:36,260 the water stops at these hydrophobic lines. 10 00:00:36,260 --> 00:00:39,070 This measures across about 4 millimeters. 11 00:00:39,070 --> 00:00:41,190 So these are drops of water that are 12 00:00:41,190 --> 00:00:44,290 taking on the shape of the hydrophobic lines. 13 00:00:44,290 --> 00:00:49,710 And this is how it was used in the article that was accepted. 14 00:00:49,710 --> 00:00:52,670 I luckily came across it, because this sort of 15 00:00:52,670 --> 00:00:57,010 started my whole career 20 years ago in science imaging. 16 00:00:57,010 --> 00:00:59,850 What I did with this image, as you've seen before, 17 00:00:59,850 --> 00:01:03,838 is I asked them to please make a triangle so I could start 18 00:01:03,838 --> 00:01:05,129 working a little bit with this. 19 00:01:05,129 --> 00:01:09,750 And I dropped some water on that triangle, also etched out. 20 00:01:09,750 --> 00:01:12,710 Here, as you remember, I made the picture 21 00:01:12,710 --> 00:01:13,760 with direct lighting. 22 00:01:13,760 --> 00:01:16,500 Here I diffused the lighting. 23 00:01:16,500 --> 00:01:18,570 And I was playing around a little more. 24 00:01:18,570 --> 00:01:21,310 Another chip was etched out as well, 25 00:01:21,310 --> 00:01:28,490 and I started using fluorescing material to drop on the chip. 26 00:01:28,490 --> 00:01:32,190 This gold, it's called a self-assembled monolayer. 27 00:01:32,190 --> 00:01:35,600 In this image, I wanted to show a sense of scale, 28 00:01:35,600 --> 00:01:39,740 so I used the syringe with which I 29 00:01:39,740 --> 00:01:41,930 dropped the water on the surface, 30 00:01:41,930 --> 00:01:44,160 just to give you a sense of scale. 31 00:01:44,160 --> 00:01:48,950 Finally, getting around to submitting for a cover design, 32 00:01:48,950 --> 00:01:51,470 I asked the researchers, could you please 33 00:01:51,470 --> 00:01:55,530 at least give me a grid of the etched lines 34 00:01:55,530 --> 00:02:01,940 so I could then drop onto the grid fluorescing green water 35 00:02:01,940 --> 00:02:05,050 and fluorescing blue water? 36 00:02:05,050 --> 00:02:07,750 And you could actually see the etched marks 37 00:02:07,750 --> 00:02:10,820 creating the hydrophobic lines. 38 00:02:10,820 --> 00:02:13,810 I didn't like seeing it too much. 39 00:02:13,810 --> 00:02:17,300 Those lines were in the way, so just moving the camera ever 40 00:02:17,300 --> 00:02:21,079 so slightly gave me a cleaner image. 41 00:02:21,079 --> 00:02:25,490 And again, I was imagining the journal's logo at the top. 42 00:02:25,490 --> 00:02:29,620 What was really important was that the editors 43 00:02:29,620 --> 00:02:34,560 felt that the way I photographed it was misinformational. 44 00:02:34,560 --> 00:02:40,740 That is, you're seeing two tones of green and two tones of blue, 45 00:02:40,740 --> 00:02:44,620 and that was miscommunication for them. 46 00:02:44,620 --> 00:02:47,020 It turns out that's really the way it was. 47 00:02:47,020 --> 00:02:49,790 But in the end, what we did decide 48 00:02:49,790 --> 00:02:53,040 to do was give them a simplified version, 49 00:02:53,040 --> 00:02:59,910 where I focused in tightly onto the square drops of water, 50 00:02:59,910 --> 00:03:02,750 as we call them. (They're colored water with fluorescing 51 00:03:02,750 --> 00:03:07,620 dyes.) Each square is about 4 millimeters across. 52 00:03:07,620 --> 00:03:12,790 And it was a simplified image, as what we strive for. 53 00:03:12,790 --> 00:03:17,170 And in the end, we were lucky, and it did get the cover.