Labs

WARNING NOTICE

The experiments described in these materials are potentially hazardous and require a high level of safety training, special facilities and equipment, and supervision by appropriate individuals. You bear the sole responsibility, liability, and risk for the implementation of such safety procedures and measures. MIT shall have no responsibility, liability, or risk for the content or implementation of any of the material presented.

Legal Notice

Background

Guidelines for Keeping a Laboratory Notebook (PDF)

Experimental Design (PDF)

Good Tissue Culture Room Practice (PDF)

Protocols

One-step RT-PCR (PDF)

Two-step RT-PCR (PDF)

Rneasy Prep of S2 RNA (PDF)

S2 Cell Culture (PDF)

In vitro Transcription (PDF)

Fungene Transcription (PDF)

Basic Bioinformatics (PDF)

dsRNA Soaking (PDF)

dsRNA Transfection (serum starvation) (PDF)