WARNING NOTICE
The experiments described in these materials are potentially hazardous and require a high level of safety training, special facilities and equipment, and supervision by appropriate individuals. You bear the sole responsibility, liability, and risk for the implementation of such safety procedures and measures. MIT shall have no responsibility, liability, or risk for the content or implementation of any of the material presented.
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Background
Guidelines for Keeping a Laboratory Notebook (PDF)
Experimental Design (PDF)
Good Tissue Culture Room Practice (PDF)
Protocols
One-step RT-PCR (PDF)
Two-step RT-PCR (PDF)
Rneasy Prep of S2 RNA (PDF)
S2 Cell Culture (PDF)
In vitro Transcription (PDF)
Fungene Transcription (PDF)
Basic Bioinformatics (PDF)
dsRNA Soaking (PDF)
dsRNA Transfection (serum starvation) (PDF)