Readings | Sweet Discoveries: Unraveling the Complex World of Sugars in Health and Disease | Biology

Course readings.
WEEK #	TOPICS	READINGS
1	Introduction See the Lecture Summary
2	Aberrant protein glycosylation in human disease See the Lecture Summary	Losfeld, M. E., F. Soncin, et al. "A Sensitive Green Fluorescent Protein Biomarker of N-glycosylation Site Occupancy." FASEB Journal 26, no. 10 (2012): 4210–17. Mir, A., N. Gao, et al. "A Zebrafish Model of Congenital Disorders of Glycosylation with Phosphomannose Isomerase Deficiency Reveals an Early Opportunity for Corrective Mannose Supplementation." Disease Models & Mechanisms 6, no. 1 (2013): 95–105.
3	Imaging glycan patterns in developing embryos See the Lecture Summary	Baskin, J. M., K. W. Dehnert, et al. "Visualizing Enveloping Layer Glycans during Zebrafish Early Embryogenesis." Proceedings of the National Academy of Sciences 107, no. 23 (2010): 10360–65. Beahm, B. J., K. W. Dehnert, et al. "A Visualizable Chain-terminating Inhibitor of Glycosaminoglycan Biosynthesis in Developing Zebrafish." Angewandte Chemie International Edition 53, no. 13 (2014): 3347–52.
4	The role of protein glycosylation in bacterial motility See the Lecture Summary	Ewing, C. P., E. Andreischcheva, et al. "Functional Characterization of Flagellin Glycosylation in Campylobacter Jejuni 81–176." Journal of Bacteriology 191, no. 22 (2009): 7086–93. Liu, F., A. J. Aubry, et al. "The Engineering of Bacteria Bearing Azido-pseudaminic Acid-modified Flagella." Chembiochem 10, no. 8 (2009): 1317–20.
5	Engineering bacteria for glycoprotein production See the Lecture Summary	Wacker, M., D. Linton, et al. "N-linked Glycosylation in Campylobacter Jejuni and its Functional Transfer into E. coli." Science 298, no. 5599 (2002): 1790–93. Valderrama-Rincon, J. D., A. C. Fisher, et al. "An Engineered Eukaryotic Protein Glycosylation Pathway in Escherichia coli." Nature Chemical Biology 8, no. 5 (2012): 434–6.
6	Chemical strategies for glycoprotein production See the Lecture Summary	Hang, Z., J. Gildersleeve, et al. "A New Strategy for the Synthesis of Glycoproteins." Science 303, no. 5656 (2004): 371–3. Van Kasteren, Sander I, H. B. Kramer, et al. "Expanding the Diversity of Chemical Protein Modification Allows Post-translational Mimicry." Nature 446, no. 7139 (2007): 1105–9.
7	Development and application of glycan arrays See the Lecture Summary	Blixt, O., S. Head, et al. "Printed Covalent Glycan Array for Ligand Profiling of Diverse Glycan Binding Proteins." Proceedings of the National Academy of Sciences 101, no. 49 (2004): 17033–8. Hung, T. -C., C.-W. Lin, et al. "Investigation of SSEA-4 Binding Protein in Breast Cancer Cells." Journal of the American Chemical Society 135, no. 16 (2013): 5934–7.
8	Metabolic glycan labeling in cancer See the Lecture Summary	Neves, A. A., H. Stöckmann, et al. "Imaging Cell Surface Glycosylation in Vivo using "Double Click" Chemistry." Bioconjugate Chemistry 24, no. 6 (2013): 934–41. Furumoto, S., R. Shinbo, et al. "In Vitro and in Vivo Characterization of 2-deoxy-2-₁₈F-fluoro-D-mannose as a Tumor-imaging Agent for PET." Journal Nuclear Medicine 54, no. 8 (2013): 1354–61.
9	Glycan-based synthetic vaccines See the Lecture Summary	Broecker, F., J. Aretz, et al. "Epitope Recognition of Antibodies against a Yersinia Pestis Lipopolysaccharide Trisaccharide Component." ACS Chemical Biology 9, no. 4 (2014): 867–73. Lakshminarayanan, V., P. Thompson, et al. "Immune Recognition of Tumor-associated Mucin MUC1 is Achieved by a Fully Synthetic Aberrantly Glycosylated MUC1 Tripartite Vaccine." Proceedings of the National Academy of Sciences 109, no.1 (2012): 261–6.
10	A close look at influenza–host interactions See the Lecture Summary	Zhang, W., Y. Shi, et al. "An Airborne Transmissible Avian Influenza H5 Hemagglutinin seen at the Atomic Level." Science 340, no. 6139 (2013): 1463–7. Connaris, H., E. A. Govorkova, et al. "Prevention of Influenza by Targeting Host Receptors using Engineered Proteins." Proceedings of the National Academy of Sciences 111, no. 17 (2014): 6401–6.
11	Synthetic antigens to generate HIV vaccines See the Lecture Summary	Wang, S. -K., P. -H. Liang, et al. "Targeting the Carbohydrates on HIV-1: Interaction of Oligomannose Dendrons with Human Monoclonal Antibody 2G12 and DC-SIGN." Proceedings of the National Academy of Sciences 105, no. 10 (2007): 3690–95. Doores, K. J., Z. Fulton, et al. "A Nonself Sugar Mimic of the HIV Glycan Shield Shows Enhanced Antigenicity." Proceedings of the National Academy of Sciences 107, no. 40 (2010): 17107–12.
12	The role of sugar metabolites in diabetes See the Lecture Summary	Clark, P. M., J. F. Dweck, et al. "Direct In-gel Fluorescence Detection and Cellular Imaging of O-GlcNAc-modified Proteins." Journal of the American Chemical Society 130, no. 35 (2008): 11576–7. Wang, Z., K. Park, et al. "Site-specific GlcNAcylation of Human Erythrocyte Proteins – Potential Biomarker(s) for Diabetes." Diabetes 58, no. 2 (2009): 309–17.
13	Presentations See the Lecture Summary	No Readings